Manipulation of spermatogonial stem cells in livestock species
نویسندگان
چکیده
منابع مشابه
generation of in-vitro spermatogonial stem cells following genetic manipulation of primordial germ-like cells
research about potential use of stem cells for the development of germ line cells in vitro had been challenged. in the present study, we reported a novel protocol consisting of cocktail growth factor addition for germ cell differentiation followed by transfection. the cells were purificated based on the expression on the cell surface of a protein. this protein is not present in normal cells of ...
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Spermatogonial stem cells (SSCs) are the only stem cells in adults that can transfer genetic information to the future generations. Considering the fact that a single SSC gives rise to a vast number of spermatozoa, genetic manipulation of these cells is a potential novel technology with feasible application to various animal species. The aim of this study was to evaluate enhanced green fluoresc...
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Background: As condition and component of culture determine fate map of spermatogonial stem cells (SSCs), the aim of this study was to evaluate of growth factors GDNF, LIF and RA on proliferation and differentiation of SSC. Materials and Methods: SSCs were cultured in two groups: The first group GDNF and LIF and the second group RA. The number of clumps and colony formation was monitored dur...
متن کاملGeneration of In-vitro Spermatogonial Stem Cells following Genetic Manipulation of Primordial Germ-like Cells
Research about potential use of stem cells for the development of germ line cells in vitro had been challenged. In the present study, we reported a novel protocol consisting of cocktail growth factor addition for germ cell differentiation followed by transfection. The cells were purificated based on the expression on the cell surface of a protein. This protein is not present in normal cells of ...
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ژورنال
عنوان ژورنال: Journal of Animal Science and Biotechnology
سال: 2019
ISSN: 2049-1891
DOI: 10.1186/s40104-019-0355-4